GLUD1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	This antibody has been tested for use in ELISA and by Western blot. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect a band approximately 62 kDa in size corresponding to glutamate dehydrogenase monomer subunit by Western blotting in the appropriate cell or tissue extract. Recommended Dilutions: ELISA: 1/4,000-1/16,000 Western blot: 1/1,000-1/3,000.
Reactivity	Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	A full length Glutamate Dehydrogenase protein isolated from Bovine Liver.
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. State: Serum State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Gene Name	glutamate dehydrogenase 1
Database Link	UniProt ID P00367
Background	Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1; EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.
Synonyms	GLUD1, GLUD, GDH1
Note	Protein sequence: Bovine GDH, 558 aa, predicted MW 61.5 kDa 1 myrylgeall lsragpaalg sasadsaall gwargqpaaa pqpglvppar rhyseaaadr 61 eddpnffkmv egffdrgasi vedklvedlk treteeqkrn rvrsilriik pcnhvlslsf 121 pirrddgswe viegyraqhs qhrtpckggi rystdvsvde vkalaslmty kcavvdvpfg 181 gakagvkinp knytdnelek itrrftmela kkgfigpgvd vpapdmstge remswiadty 241 astighydin ahacvtgkpi sqggihgris atgrgvfhgi enfineasym silgmtpgfg 301 dktfvvqgfg nvglhsmryl hrfgakciav gesdgsiwnp dgidpkeled fklqhgtilg 361 fpkakiyegs ilevdcdili paasekqlpk snaprvkaki iaegangptt peadkifler 421 nimvipdlyl naggvtvsyf ewlnnlnhvs ygrltfkyer dsnyhllmsv qeslerkfgk 481 hggtipivpt aefqdrisga sekdivhsgl aytmersarq imrtamkynl gldlrtaayv 541 naiekvfrvy neagvtft
Reference Data

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