GOT1 Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/5,000-1/25,000. Western Blot: 1/500-1/3,000. This product has been assayed against 1.0 µg of Aspartate Aminotransferase (AST) [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified Goat anti-Sheep IgG [H&L] (R1364HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/16,000 of the reconstitution concentration is suggested for this product.
Reactivity	Porcine
Host	Sheep
Clonality	Polyclonal
Immunogen	Aspartate Aminotransferase (AST) from Porcine Heart.
Specificity	Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart]. Cross reactivity against Aspartate Aminotransferase (AST) from other tissues and species may occur but have not been specifically determined.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide as preservative. State: Serum State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by a Delipidation and Defibrination.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P00503
Background	Aspartate Aminotransferase [Glutamate oxaloacetate transaminase] is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.
Synonyms	Aspartate aminotransferase, Transaminase A
Note	Aspartate Aminotransferase (AST) is also referred to as Glutamic Oxaloacetic Transaminase (GOT).
Reference Data

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