GOT1 Sheep Polyclonal Antibody
CNY 20,681.00
货期*
4周
规格
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommend Dilution | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/5,000-1/25,000. Western Blot: 1/500-1/3,000. This product has been assayed against 1.0 µg of Aspartate Aminotransferase (AST) [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified Goat anti-Sheep IgG [H&L] (R1364HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/16,000 of the reconstitution concentration is suggested for this product. |
Reactivity | Porcine |
Host | Sheep |
Clonality | Polyclonal |
Immunogen | Aspartate Aminotransferase (AST) from Porcine Heart. |
Specificity | Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart]. Cross reactivity against Aspartate Aminotransferase (AST) from other tissues and species may occur but have not been specifically determined. |
Formulation | 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide as preservative. State: Serum State: Lyophilized purified Ig fraction. |
Reconstitution Method | Restore with 2.0 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Prepared from monospecific antiserum by a Delipidation and Defibrination. |
Conjugation | Unconjugated |
Storage Condition | Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. |
Database Link | |
Background | Aspartate Aminotransferase [Glutamate oxaloacetate transaminase] is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower. |
Synonyms | Aspartate aminotransferase, Transaminase A |
Note | Aspartate Aminotransferase (AST) is also referred to as Glutamic Oxaloacetic Transaminase (GOT). |
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