GOT1 Sheep Polyclonal Antibody
CAT#: R1087HRPS
GOT1 sheep polyclonal antibody, HRP, Purified
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CNY 7,688.00
货期*
4周
规格
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | Western blot: 1/1,000-1/5,000. ELISA: 1/10,000-1/40,000. This antibody has been assayed against 1.0 µg of aspartate transaminase [porcine heart] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested. |
Reactivity | Porcine |
Host | Sheep |
Clonality | Polyclonal |
Immunogen | Aspartate aminotransferase / GOT1 from porcine heart |
Specificity | This antibody detects porcine GOT1. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase and anti-sheep serum as well as purified and partially purified aspartate transaminase / GOT1 [porcine heart]. |
Formulation | 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | HRP |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Database Link | |
Background | Aspartate aminotransferase (Glutamate oxaloacetate transaminase / GOT1) is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT (with SGPT) levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower. |
Synonyms | Aspartate aminotransferase, Transaminase A |
Note | Aspartate aminotransferase (AST) is also referred to as glutamic oxaloacetic transaminase (GOT). |
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