PKM (pan m-PK) Goat Polyclonal Antibody

CAT#: R1108BS

PKM (pan m-PK) goat polyclonal antibody, Biotin, Purified



Need it in bulk or conjugated?
Get a free quote

CNY 7,688.00


货期*
4周

规格
    • 100 ug

Product images

Specifications

Product Data
Applications ELISA, WB
Recommend Dilution Western blot: 1/500-1/2,000.
ELISA
: 1/4,000-1/20,000.
This product has been assayed against 1.0 µg of Pyruvate Kinase [rabbit muscle] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,000 to 1/15,000 of the reconstitution concentration is suggested.
Reactivity Rabbit
Host Goat
Clonality Polyclonal
Immunogen Pyruvate Kinase from rabbit muscle
Specificity This antibody detects rabbit Pyruvate Kinase. Cross reactivity may occur against other mammalian Pyruvate Kinase, but were not specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-goat serum as well as  purified and partially purified Pyruvate Kinase [rabbit muscle].
Formulation 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Label: Biotin
State: Purified
State: Lyophilized purified Ig fraction
Stabilizer: 10 mg/m BSA (immunoglobulin and protease free)
Preservative: 0.01% Sodium azide
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration lot specific
Purification Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation Biotin
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Background Pyruvate Kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Genetic defects of this enzyme cause the disease known as pyruvate kinase deficiency and can cause hemolytic anemia.
Synonyms PK2, PK3, PKM, CTHBP, M2-PK, THBP1, OIP3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase muscle
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...