RNASE1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western Blot : 1/500 - 1/2,500. Immunoprecipitation: 1/100. ELISA : 1/2,500 - 1/10,000. This product has been assayed against 1.0 µg of Ribonuclease A [bovine pancreas] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,000 to 1/13,000 is suggested.
Reactivity	Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Ribonuclease A from bovine pancreas
Specificity	This antibody detects bovine RNASE1. Cross reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Ribonuclease A [bovine pancreas].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/m BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 282340 Bovine UniProt ID P61823
Synonyms	Ribonuclease 1, RNase 1, Ribonuclease A, RNase A, RIB1, RIB-1, RNS1, Ribonuclease pancreatic
Reference Data

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