Trypsinogen Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Western blot: 1/500-1/3,000. ELISA: 1/5,000-1/25,000. This product has been assayed against 1.0 µg of Trypsinogen [bovine pancreas] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested for this product.
Reactivity	Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Trypsinogen from bovine pancreas
Specificity	This product detects bovine Trypsinogen. Cross reactivity against Trypsinogen from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-rabbit serum as well as purified and partially purified Trypsinogen [bovine pancreas].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.