ABC3735 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot: 1/500-1/1000. ELISA: 1/1000-1/2500; This antibody has been assayed against 1.0 ug of uricase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. Immunoprecipitation: 1:100.
Reactivity	Bacillus sp.
Host	Goat
Clonality	Polyclonal
Immunogen	Uricase from Bacillus species
Specificity	This Antibody detects Uricase [Bacillus species]. Cross reactivity against uricase from other sources is unknown. Immunoelectrophoresis gives a single precipitin arc against anti-Biotin, anti-goat serum as well as purified and partially purified uricase [Bacillus species].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q5WBJ3
Background	Uricase is a peroxisomal enzyme that catalyzes the conversion of urea to allantoin. Uricase performs this action by cleaving the purine ring of uric acid rendering it much more soluble within the body for excretion. Uricase within the Bacillus species is of high importance due to its high activity and thermostability over a wide range of pH's.
Synonyms	URIC, URO, UOX, EC 1.7.3.3, Urate oxidase
Reference Data

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