ACHE Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	ELISA: 1/5,000-1/20,000. This antibody has been assayed against 1.0 µg of acetylcholinesterase [bovine erythrocytes] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000-1/2,000 of the reconstitution concentration is suggested. Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100.
Reactivity	Bovine
Host	Goat
Clonality	Polyclonal
Immunogen	Acetylcholinesterase from bovine erythrocytes
Specificity	This antibody detects bovine acetylcholinesterase. Cross reactivity against acetylcholinesterase from other sources is unknown. Immunoelectrophoresis gives a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified acetylcholinesterase [bovine erythrocytes].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent)
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 540446 Bovine UniProt ID P23795
Synonyms	AChE, Acetylcholine Esterase, Acetylcholine-Esterase
Reference Data

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