ycjM Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of Sucrose Phosphorylase [E.coli] in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/25,500 to 1/115,000 of the reconstitution concentration is suggested for this product.
Reactivity	Escherichia coli
Host	Goat
Clonality	Polyclonal
Immunogen	Sucrose phosphorylase from E.coli
Specificity	This antibody detects E.coli Sucrose phosphorylase. Cross reactivity against Sucrose phosphorylase from other sources is unknown. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified Sucrose phosphorylase [E.coli].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/m BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P76041
Synonyms	Sucrose glucosyltransferase (E. coli)
Reference Data

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