HUB1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	This purified polyclonal antibody reacts with yeast Hub1 by Western blot and ELISA. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions. A 9.7 kDa band corresponding to yeast Hub1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/500-1/2,000. Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation.
Reactivity	Yeast
Host	Rabbit
Clonality	Polyclonal
Immunogen	This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Hub1 protein.
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 0.1 ml of deionized water or equivalent.
Concentration	lot specific
Purification	A multi-step process which includes Delipidation, Salt Fractionation and Ion exchange chromatography followed by extensive dialysis against the buffer stated below.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q6Q546
Background	Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Hub1 ("Homologous to UBiquitin") may function as a modifier (see figure 2) but its role is unclear because it lacks the double glycine motif characteristic for ubiquitin and ubiquitin-like modifiers. Recently cell polarity factors Sph1 and Hbt1 have been identified as in vivo targets of Hub1 conjugation. HUB1 has close homologs in other species including humans. The human homologs is called Ubl5.
Synonyms	YNR032C-A
Reference Data

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