URM1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Suitable for ELISA (1/2,000-1/10,000) and Immunoblotting (1/1,000). Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. A 12 kDa band corresponding to yeast Urm1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.
Reactivity	Yeast
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant yeast Urm1 protein.
Specificity	This product is prepared from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% (w/v) Sodium Azide as preservative. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Database Link	UniProt ID P40554
Background	Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Urm1 is a newly identified ubiquitin related modifier. Urm 1 is a 99-amino acid protein terminated with glycine-glycine. Target proteins are conjugated to Urm1 via its C-terminal glycine. Initially Urm1 forms a thioester with a novel E1-like protein, Uba4.
Synonyms	URM-1, Ubiquitin-related modifier 1, C9orf74, YIL008W
Reference Data

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