Ferret IgG (gamma chain specific) Goat Polyclonal Antibody

Specifications

Product Data
Recommend Dilution	This product is designed for Immunofluorescence Microscopy, fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions FLISA: 1/10,000-1/50,000. IF Microscopy: 1/1,000-1/5,000. Flow Cytometry: 1/500-1/2,500.
Reactivity	Ferret
Host	Goat
Clonality	Polyclonal
Immunogen	Ferret IgG γ gamma chain.
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Ferret IgG and Ferret Serum. No reaction was observed against Ferret IgA or Ferret IgM. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Ferret heavy or light chain isotypes.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: FITC State: Lyophilized purified Ig fraction. Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons) Absorption emission: 495 nm / 528 nm Molar radio: 4.0 moles FITC per mole of Goat IgG.
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Immunoaffinity Chromatography.
Conjugation	FITC
Storage Condition	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Synonyms	Ferret Immunoglobulin G
Reference Data

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