Human Lambda Light Chain (F(ab)2 Fragment) Goat Polyclonal Antibody

Specifications

Product Data
Recommend Dilution	Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Human lambda light chain
Specificity	This antibody detects Human Lambda Light chain. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Formulation	10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer; 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 as buffer; 0.01% (w/v) Sodium Azide as preservative Label: Texas Red State: Lyophilized F(ab)2 Fragment Label: Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption emission: 596 nm / 620 nm Molar radio: 1.6 moles Texas RedTM per mole of Goat IgG F(ab')2
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent). Dilute only prior to immediate use.
Concentration	lot specific
Purification	Prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation	Texas Red
Storage Condition	Store vial at 2-8 °C prior to restoration. For extended storage following reconstitution add glycerol to 50% and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for four weeks at 2-8 °C as an undiluted liquid.
Reference Data

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