Glycogen synthase 1 (GYS1) pSer641 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	This phospho specific polyclonal antibody was tested by Immunoblotting and ELISA. By ELISA the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting will detect human and mouse muscle glycogen synthase. Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature. A working dilution of 1:30,000 to 1:150,000 is suggested for this product. Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide. Dilute the antibody 1:1,000 for Immunoblotting.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Human Muscle Glycogen Synthase phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity	This affinity purified antibody is directed against human muscle glycogen synthase. The product was affinity purified from monospecific antiserum by Immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS640 of human muscle glycogen synthase. Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA. Cross reactivity with muscle glycogen synthase occurs in mouse tissue. Reactivity with muscle glycogen synthase from other sources has not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. State: Aff - Purified State: Liquid (sterile filtered) purified Ig fraction.
Concentration	lot specific
Purification	Immunoaffinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Gene Name	glycogen synthase 1
Database Link	Entrez Gene 14936 MouseEntrez Gene 2997 Human UniProt ID P13807
Background	Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3β that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S640. Phosphorylation of GS at S640 has been associated with Antiphospholipid Antibody Syndrome.
Synonyms	GYS1, GYS
Reference Data
Protein Pathways	Insulin signaling pathway, Starch and sucrose metabolism

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