P2X2 (P2RX2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunohistochemistry: 1:1000. Immunocytochemistry: 1:1000. Western Blot: 1:1000.
Reactivity	Human, Monkey, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Corresponding to residues 460-472 of the carboxy-terminus of rat P2X2.
Specificity	This antibody reacts to P2X2.
Formulation	State: Serum State: Liquid serum containing 0.05% sodium azide
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: 6 month from despatch.
Gene Name	purinergic receptor P2X 2
Database Link	Entrez Gene 22953 Human UniProt ID Q9UBL9
Synonyms	P2X purinoceptor 2, ATP receptor, Purinergic receptor
Note	Sodium azide (NaN3) interferes with peroxidase reactions and should not be used with peroxidase methodologies. If sodium azide is present in any steps of the staining procedure, the tissue should thoroughly be rinsed with sodium azide-free buffer before performing the peroxidase reaction. Protocol: Immunohistochemistry: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrodes solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. Immunocytochemistry: P2X2 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above. Western Blotting: Cell membrane extracts were examined by electrophoresis (8% acrylamide) with SDS under reducing conditions and transferred to a nylon membrane. Membranes were blocked for 1 hour at 4°C with 0.1% Tween 20 and 2.5% milk powder (w/v) in PBS. Membranes were incubated with primary antiserum (1:1000) in the same buffer overnight at 4°C. Membranes were rinsed and incubated with horseradish peroxidase conjugated secondary antibody for 1 hour at room temperature. Following rinsing, the membranes were processed using enhanced chemiluminescence.
Reference Data

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