Cd8a Rat Monoclonal Antibody [Clone ID: KT15]

Specifications

Product Data
Clone Name	KT15
Applications	FC, IF, IHC
Recommend Dilution	Flow cytometry: 1/10 - 1/50, use 10 µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on frozen sections.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	T cell clone, C6. Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.
Specificity	This antibody recognises mouse Ly2.1 and Ly2.2 cells; reacting with the CD8 alpha chain (non polymorphic). It is weakly cytotoxic.
Formulation	PBs, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD8 antigen, alpha chain
Database Link	Entrez Gene 12525 Mouse UniProt ID P01731
Background	The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
Synonyms	CD8 alpha chain, CD8A, MAL
Reference Data

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