CD45 / LCA Rat Monoclonal Antibody [Clone ID: YW 62.3]

Specifications

Product Data
Clone Name	YW 62.3
Applications	FC
Recommend Dilution	Flow cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse spleen cells. Spleen cells from immunised DA rat were fused with cells of the Y3/Ag1.2.3 myeloma cell line.
Specificity	This antibody recognises the CD45 cell surface antigen, a 180-220kD glycoprotein expressed by all leucocytes.
Formulation	PBS containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Gene Name	protein tyrosine phosphatase, receptor type, C
Database Link	Entrez Gene 19264 Mouse UniProt ID P06800
Background	CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.