Integrin alpha 4 (ITGA4) Rat Monoclonal Antibody [Clone ID: PS/2]

Specifications

Product Data
Clone Name	PS/2
Applications	FC, FN, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl. Immunohistochemistry on frozen sections. Immunoprecipitation. Functional assays.
Reactivity	Human, Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This antibody recognises alpha 4 integrin (CD49d). Clone PS/2 has also been reported to block the binding of CD49d to its ligands.
Formulation	PBS, pH 7.4 State: Low Endotoxin State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha 4
Database Link	Entrez Gene 3676 Human UniProt ID P13612
Background	Integrin α4 (also called CD49d) is a ~150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin α4 forms heterodimers with integrins β1 and β7. Integrin α4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin α4 promotes cell migration and inhibits cell spreading and contractility. Integrin α4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin α4 function has become an important target for drug discovery. Phosphorylation of the serine 988 residue on the integrin α4 cytoplasmic tail inhibits its interaction with the focal adhesion complex-associated protein paxillin, and modulates its effects on cell motility and spreading.
Synonyms	Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4
Reference Data

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