Thy1 Mouse Monoclonal Antibody [Clone ID: OX-7]

Specifications

Product Data
Clone Name	OX-7
Applications	FC
Recommend Dilution	Flow Cytometry:  Use 10 µl of neat antibody to label 106 cells in 100 µl.  This product is routinely tested in flow cytometry on Rat thymocytes.
Reactivity	Guinea Pig, Mouse, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat Thy 1 antigen. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognizes CD90, also known as Thy1.1,  expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues. Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using fluorescence microscopy. Affinity Constant of the Fab' of MRC OX-7 for:  Rat: 3 x 109m-1 Mouse: 3 x 108m-1  Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using Fluorescence Microscopy, See Ref.5 for details. This clone has been reported to induce glomerular nephritis in Wistar rats, See Ref.8 for details.
Formulation	PBS Label: PE State: Lyophilized purified IgG fraction Stabilizer: 1% BSA and 5% Sucrose Preservative: 0.09% Sodium Azide Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with distilled water.
Purification	Ion Exchange Chromatography
Conjugation	PE
Storage Condition	Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	thymus cell antigen 1, theta
Database Link	Entrez Gene 21838 Mouse UniProt ID P01831
Background	CD90 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues.
Synonyms	Thy-1, THY1, CDw90
Reference Data

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