Siglec1 Rat Monoclonal Antibody [Clone ID: MOMA-1]
CAT#: SM066P
Siglec1 rat monoclonal antibody, clone MOMA-1, Purified
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CNY 9,600.00
货期*
5周
规格
Cited in 1 publication. |
Specifications
Product Data | |
Clone Name | MOMA-1 |
Applications | FC, IHC |
Recommend Dilution | Flow Cytometry. Immunohistochemistry on Frozen Sections: 2 µg/ml (1/200). Fixation: Acetone. Recommended Positive Control: Mouse spleen. Does not react on routinely processed Paraffin Sections. |
Reactivity | Mouse |
Host | Rat |
Clonality | Monoclonal |
Immunogen | Mouse lymph node tissue. |
Specificity | Clone MOMA-1 is a useful marker for the identification of Macrophage subpopulations in various organs, mostly characterised by a high level of non-specific esterase expression. The staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. The marker is also very suitable for differentiation of non-metallophilic marginal zone Macrophages as detected by ER-TR9 (Cat.-No BM4011). In addition , MOMA-1 detects Macrophages at inflammatory sites and is positive with Kupffer cells. The antigen is differentially induced in in vitro derived Macrophages depending on the colony-stimulating factor applied (IL-3 > M-CSF > GM-CSF). The antigen detected by MOMA-1 is CD169, also known as Sialoadhesin. Antigen Distribution Isolated Cells: No reactivity of MOMA-1 was found with dendritic cells, peritoneal resident Macrophages, peritoneal exudate cells, bone marrow or blood cells. Tissue Sections: Distinct Macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer's patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1. No MOMA-1-positive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a Macrophage subpopulation in the lamina propria of the villi of the small intestine. |
Formulation | Stock solutions contains PBS, pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.01% Thimerosal as a preservative State: Purified State: Lyophilized purified IgG fraction |
Reconstitution Method | Restore by adding 0.5 ml distilled water (= 0.4 mg/ml Stock Solution). |
Concentration | 0.4 mg/ml (after reconstitution) |
Purification | Affinity Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Gene Name | sialic acid binding Ig-like lectin 1, sialoadhesin |
Database Link | |
Background | Metallophilic macrophages are a subpopulation of mature resident tissue macrophages. They show high non specific esterase activity and can be distinguished from splenic marginal zone macrophages by antibody staining and the lack of FITC-Ficoll uptake. |
Synonyms | Sialoadhesin, Siglec-1 |
Note | Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum |
Reference Data |
Citations (1)
The use of this Antibodies has been cited in the following citations: |
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Kupffer cells express a unique combination of phenotypic and functional characteristics compared with splenic and peritoneal macrophages
,Movita D, Kreefft K, Biesta P, van Oudenaren A, Leenen PJ, Janssen HL, Boonstra A,
J. Leukoc. Biol.
,PubMed ID 22685319
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