MHC Class II I-Ak Mouse Monoclonal Antibody [Clone ID: 14V.18]

CAT#: SM083FS

MHC Class II I-Ak mouse monoclonal antibody, clone 14V.18, FITC



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CNY 3,710.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 14V.18
Applications FC
Recommend Dilution Flow Cytometry (See Protocols).
Reactivity Mouse
Host Mouse
Clonality Monoclonal
Immunogen A.TL spleen.
Donor: A.TH spleen.
Fusion Partner: P3-X63-Ag8
Specificity This Monoclonal antibody is a cytotoxic antibody specific for cells expressing the Ia antigen coded for by the A subregion of the k haplotype. The reaction pattern of this antibody with a panel of inbred and recombinant haplotypes demonstrates that the antibody reacts with Ia.m2, a private specificity of the H-2k haplotype.
This antibody can be used to quantitate or to eliminate cells bearing the I-Ak (Ia.m2) antigen.
Formulation PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
Label: FITC
State: Liquid purified IgG fraction
Label: Fluorescein
Concentration lot specific
Purification Protein G Chromatography
Conjugation FITC
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Synonyms H2-Aa
Note Protocol: FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 106 cells, representing 1 test).
4. To each tube, add 1.0 μg* of SM083FS per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
(It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.)
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 μl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium
azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide
in 100 mls).

Results:
Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: A.TL
Cell Concentration : 1x10e6 cells per test.
Antibody Concentration Used: 1.0 μg/106 cells.
Isotypic Control: FITC Mouse IgG2a

Cell Source Percentage of cells stained above control:
Thymus: 32.5%
Spleen: 75.4%
Lymph Node: 32.1%
Bone Marrow: 21.6%

Strain Distribution by Flow Cytometry Analysis:

Cell Concentration : 1x10e6 cells per test.
Antibody Concentration Used: 1.0 μg /10e6 cells.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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