Ifna1 Rat Monoclonal Antibody [Clone ID: F18]

Specifications

Product Data
Clone Name	F18
Applications	ELISA, FC, FN
Recommend Dilution	Flow Cytometry: For intracellular staining of IFN-Alpha, cells can be fixed in 1% formaldehyde; blocked and permeabilized in 0.2% Saponin, 5% normal Rabbit serum for 30 minutes on ice (Ref.2). The typical starting working dilution is 1/10. Functional Assays: Neutralization of IFN-α by adding 1 µg antibody F18 per Mouse i.v. before treatment with 35 µg LPS i.p., decreased the LPS-induced IL-1β serum response (Ref.3). Immunoassays (Ref.1). Western Blot. Positive Control: Plasmacytoid dendritic cells.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This antibody recognises most isoforms of natural and recombinant alpha Interferon. It does not cross-react with Murine beta or gamma Interferon, nor with Human alpha Interferon. The antibody exhibits neutralizing activity of approximately 6.25 x 10e4 neutralizing units/mg.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	interferon alpha 1
Database Link	Entrez Gene 15962 Mouse UniProt ID P01572
Background	The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. Interferon alpha is produced primarily by plasmacytoid dendritic cells and is a potent component of the anti viral innate immune response; it modulates adaptive immunity. The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent.
Synonyms	IFNA13, Interferon alpha-D, LeIF D, IFN-alpha 1
Reference Data

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