CD3E Mouse Monoclonal Antibody [Clone ID: UCHT1]

Specifications

Product Data
Clone Name	UCHT1
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/100-1/200 diluted antibody to label 10e6 cells or 100µl of lysed whole peripheral blood. Immunohistochemistry on Frozen Sections.
Reactivity	Human, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome. Spleen cells from immunised BALB/c mice were fused with cells of the P3/NS1/1-Ag4-1 mouse myeloma cell line.
Specificity	This antibody recognises a surface protein of peripheral blood T cells of molecular weight 19kD. As a pan T cell marker this antibody is present on more than 95% of circulating human peripheral T cells.
Formulation	State: Purified State: Liquid purified IgG containing 0.09% Sodium Azide as preservative.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD3e molecule
Database Link	Entrez Gene 916 Human UniProt ID P07766
Background	T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Synonyms	T3/Leu-4
Reference Data

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