CD18 (ITGB2) Rat Monoclonal Antibody [Clone ID: YFC118.3]

Specifications

Product Data
Clone Name	YFC118.3
Applications	FC
Recommend Dilution	Flow cytometry: Use 10 µl of neat antibody to label 100 µl whole blood or 10e6 cells in 100 µl.
Reactivity	Canine, Human
Host	Rat
Clonality	Monoclonal
Immunogen	Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the Y3/Ag.1.2.3 rat myeloma cell line.
Specificity	YFC118.3 was clustered at the Fourth International Workshop on Leucocyte Differentiation Antigen as recognising the CD18 antigen. The main cellular reactivity of antibody YFC 118.3 is a strong reactivity with Leucocytes (platelets negative). YFC118.3 blocks homotypic adhesion and other lymphocyte functions which involve the LFA-1 antigen.
Formulation	PBS, pH7.2 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: PE State: Lyophilized purified IgG Label: R. Phycoerythrin (RPE)
Reconstitution Method	SM1076R: Restore with 1 ml distilled water. SM1076RT: Restore with 25 µl distilled water.
Purification	Affinity chromatography on Protein G
Conjugation	PE
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	integrin subunit beta 2
Database Link	Entrez Gene 3689 Human UniProt ID P05107
Background	The CD18 antigen is an integral membrane glycoprotein of 95kD molecular weight - known as the b chain, of the LFA-1 complex. The CD18 antigen is non-covalently linked to CD11a, b, c molecules. The CD18 molecule is the receptor for ICAM-1 and is thus important for cell adhesion.
Synonyms	Integrin beta-2, MFI7
Reference Data

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