CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: AT10]

Specifications

Product Data
Clone Name	AT10
Applications	FC, IHC, IP
Recommend Dilution	Immunoprecipitation: 20 µg/ml. Flow Cytometry: Use 10 µl of 20 µg/ml diluted antibody to label 10e6 cells or 100 µl whole blood. Immunohistochemistry on Frozen Sections: 1/500-1/1000, not suitable for paraffin sections. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. It is recommended to use acetone fixation for frozen sections. Recommended Positive Control: Lymph node.
Reactivity	Canine, Human, Monkey, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	K562 cell line.
Specificity	This antibody recognises the CD32 antigen. Clone AT10 blocks the binding of IgG to Fc gamma R11. SM1092LE is recommended for this purpose.
Formulation	PBS containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purifed IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein A.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Fc fragment of IgG receptor IIa
Database Link	Entrez Gene 2212 Human UniProt ID P12318
Background	CD32 is a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.
Synonyms	Fc-gamma RII-a, Fc-gamma-RIIa, CDw32, FCGR2A, FCG2, FCGR2A1, IGFR2
Reference Data

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