Integrin alpha 4 (ITGA4) Mouse Monoclonal Antibody [Clone ID: 44H6]

Specifications

Product Data
Clone Name	44H6
Applications	FC
Recommend Dilution	Flow cytometry: 1/40; use 10 µl of the suggested working dilution to label 100µl of human whole peripheral blood. Immunohistochemistry on frozen sections: 1/50 - 1/100; Overnight incubation recommended. Recommended positive control tissue: Human Tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	HOON pre-B leukaemia cell line. Spleen cells from immunised BALB/c mice were fused with cells of the SP2.0-Ag14 mouse myeloma cell line.
Specificity	This antibody recognises CD49d, also known as integrin alpha 4, which associates with either integrin Beta 1 (to form VLA-4) or Beta 7.
Formulation	PBS, pH7.2 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha 4
Database Link	Entrez Gene 3676 Human UniProt ID P13612
Background	CD49d is a 150kD molecule, which may be cleaved to 80kD and 70kD fragments and is expressed by lymphocytes, monocytes, eosinophils and thymocytes. VLA-4 mediates cell adhesion to VCAM-1 and fibronectin.
Synonyms	Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4
Reference Data

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