CD53 Mouse Monoclonal Antibody [Clone ID: MEM-53]

Specifications

Product Data
Clone Name	MEM-53
Applications	FC, IHC, IP, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/10-1/25 diluted antibody to label 10e6 cells in 100 µl. Western blotting (non-reducing conditions). Immunoprecipitation. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 10 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognises the CD53 cell surface antigen, a glycoprotein of 32-42kD. CD53 is expressed by all leucocytes, but is absent from erythrocytes and platelets.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD53 molecule
Database Link	Entrez Gene 963 Human UniProt ID P19397
Background	CD53 is a member of the tetraspan family of molecules, and is expressed by all leucocytes, but is absent from red cells and platelets. Most of the tetraspan family of molecules are cell surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD53 is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2 generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. CD53 is a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.
Synonyms	Cell surface glycoprotein CD53, Tetraspanin-25, MOX44, TSPAN25
Reference Data

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