CD55 / DAF Mouse Monoclonal Antibody [Clone ID: 67]
CAT#: SM1141P
CD55 / DAF mouse monoclonal antibody, clone 67, Purified
Size: 100 ug
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CNY 6,116.00
货期*
4周
规格
Cited in 2 publications. |
Specifications
Product Data | |
Clone Name | 67 |
Applications | FC, IHC |
Recommend Dilution | Flow Cytometry: Use 10 µl of 1/10-1/25 diluted antibody to label 10e6 cells in 100 µl. Immunohistochemistry on Frozen Sections: 1/100-1/1000. Recommended Positive Control Tissue: Human Tonsil. (Successful use in immunohistochemistry on paraffin embedded sections (1/50-1/100 using heat treatment for antigen retrieval) was reported by some researchers. As this detection method has not been verified by Acris Antibodies, the application was deleted from the database. This does not necessarily exclude the use in such procedure). |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | K562 cells |
Specificity | This antibody recognises the CD55 cell surface antigen. Erythrocyte expression is very weak. Please Note: We do not recommend the use of this reagent to stain Erythrocytes. |
Formulation | PBS,pH 7.4, with 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction. |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G. |
Conjugation | Unconjugated |
Storage Condition | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Gene Name | CD55 molecule (Cromer blood group) |
Database Link | |
Background | CD55 is the complement regulatory protein, decay accelerating factor (DAF). It is a 70kD glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. DAF has a wide distribution on cells in non-haemopoietic tissues, particularly epithelium and is specifically found at the foetal-maternal interface in placenta. Soluble forms of DAF are found, for example, in plasma, saliva and urine. The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin. CD55 is expressed by a wide range of cell types. |
Synonyms | CR; CROM; DAF; TC |
Reference Data |
Citations (2)
The use of this Antibodies has been cited in the following citations: |
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Increased susceptibility to complement attack due to down-regulation of decay-accelerating factor/CD55 in dysferlin-deficient muscular dystrophy
,Wenzel K, Zabojszcza J, Carl M, Taubert S, Lass A, Harris CL, Ho M, Schulz H, Hummel O, Hubner N, Osterziel KJ, Spuler S,
J. Immunol.
,PubMed ID 16237120
[CD55]
|
Complement-dependent and -independent aquaporin 4-antibody-mediated cytotoxicity in human astrocytes: Pathogenetic implications in neuromyelitis optica
,null,
Biochemistry and Biophysics Reports
,PubMed ID 29114578
[CD55]
|
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