B7-2 (CD86) Mouse Monoclonal Antibody [Clone ID: BU63]

Specifications

Product Data
Clone Name	BU63
Applications	FC, FN, IHC, IP, WB
Recommend Dilution	Flow cytometry: 5 µg/ml. Immunoprecipitation. Western blot. Immunohistochemistry on frozen sections. Functional application: Blocks mixed lymphocyte reaction (MLR) and binding of soluble CTLA-4 (CD152)-mulg fusion protein to CD86 (B7-2).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	B-lymphoblastoid cell line ARH 77
Specificity	This antibody reacts with CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Formulation	Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized State: Low Endotoxin State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD86 molecule
Database Link	Entrez Gene 942 Human UniProt ID P42081
Background	CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Synonyms	CD28LG2, Activation B7-2 antigen, B70, BU63, B7.2, FUN-1
Reference Data

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