B7-2 (CD86) Mouse Monoclonal Antibody [Clone ID: BU63]

Specifications

Product Data
Clone Name	BU63
Applications	FC, IHC, IP
Recommend Dilution	Immunoprecipitation. Flow Cytometry: 1/20-1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on Frozen Sections: 1/20-1/100. Recommended Positive Control: Tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human peripheral blood lymphocytes. Spleen cells from immunised mice were fused with cells of the P3 X63 Ag8.653 myeloma cell line.
Specificity	This antibody recognises CD86. Domain depletion epitope mapping studies indicate that the binding site of Mouse anti Human CD86, Clone Bu63 is located within the Ig-v-like domain of Human CD86 (Jeanin et al. 1997).
Formulation	0.1M Tris buffered saline, pH 8.2 State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD86 molecule
Database Link	Entrez Gene 942 Human UniProt ID P42081
Background	CD86 is a 70kD cell surface protein expressed by activated B cells and monocytes. CD86 is also known as B7-2 and is the second ligand for CD28 and CTLA-4. It may have an important role in costimulation of T cells in the primary immune response.
Synonyms	CD28LG2, Activation B7-2 antigen, B70, BU63, B7.2, FUN-1
Reference Data

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