C5R1 (C5AR1) (N-term) Mouse Monoclonal Antibody [Clone ID: S5/1]

Specifications

Product Data
Clone Name	S5/1
Applications	FC, FN, IHC
Recommend Dilution	Flow cytometry: 1/25-1/200; Use 10 µl to label 5x10e5 cells. Immunohistochemistry on Paraffin sections: This product does not require protein digestion pretreatment nor antigen retrieval using heat treatment prior to staining of paraffin sections. Functional assays. Does not work on frozen sections or in immunoprecipitation.
Reactivity	Bovine, Human, Rabbit
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide comprising the N-terminal extracellular domain (Met1 - Asn31) of the C5a receptor (C5aR) CD88. Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.
Specificity	The antibody recognises the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone S5/1 (raised against a synthetic peptide Met1 -Asn31) has recently been shown to recognise the heptameric peptide (D15DKDTLD21). Clone S5/1 inhibits C5a binding to its receptor.
Formulation	PBS without stabilizer or preservative State: Azide Free State: Liquid purified IgG
Concentration	lot specific
Purification	Protein G chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	complement component 5a receptor 1
Database Link	Entrez Gene 728 Human UniProt ID P21730
Background	The human anaphylatoxin C5a is a 74 residue glycopeptide which is generated by proteolytic cleavage of the complement factor C5 in the course of complement activation. A variety of biological effects evoked by C5a can be demonstrated, rendering this molecule an important mediartor of inflammation, with granulocytes and macrophages as the main target cells. All cellular responses to C5a are specifically mediated by interactions with the membrane bound C5a receptor, a seven transmembrane GTP binding coupled receptor that belongs to the rhodopsin supergene family. C5a receptor 1 expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. ESTs have been isolated from B cell/lung/testis, nerve, and placenta libraries.
Synonyms	C5a-R, C5aR, C5AR1, Complement Component 5a Receptor 1
Reference Data

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