Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: DC-10]

Specifications

Product Data
Clone Name	DC-10
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	ELISA: This antibody clone DC-10 has been tested as the detection antibody in a Sandwich ELISA for analysis of Cytokeratin 18 in combination with the clone C-04 (Cat.-No SM3043P). Positive Control: MCF-7 Human breast adenocarcinoma cell line.  Flow Cytometry: 1-4 µg/ml. Intracellular staining. Immunoprecipitation. Western Blotting(Non-Reducing Conditions): 1-2 µg/ml, overnight in 4°C. Positive Control: HeLa human cervix carcinoma cell line, MCF-7 human breast adenocarcinoma cell line.  Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. SDS-PAGE (10% separating gel).  Immunocytochemistry. Positive Control: MCF-7 Human breast adenocarcinoma cell line, HeLa Human cervix carcinoma cell line. Immunohistochemistry on Paraffin Sections: 10 µg/ml.  Positive tissue: Epithelial tissue (skin), colon.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human breast carcinoma cell line PMC-42
Specificity	The antibody reacts with Human Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.  Negative Species: Porcine, Mouse, Rat, Hamster, Bovine, Canine (Dog), Sheep.
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 18
Database Link	Entrez Gene 3875 Human UniProt ID P05783
Background	Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).
Synonyms	Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.