CD81 / TAPA1 Hamster Monoclonal Antibody [Clone ID: Eat2]

Specifications

Product Data
Clone Name	Eat2
Applications	ELISA, FC, IHC, IP, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/10-1/50 diluted antibody to label 10e6 cells in 100 µl. Immunoprecipitation. Immunohistochemistry on Frozen Sections. ELISA. Western Blot.
Reactivity	Mouse
Host	Hamster
Clonality	Monoclonal
Immunogen	38C13, murine B cell line. Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.
Specificity	This antibody recognises and CD81. In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. It induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD81 antigen
Database Link	Entrez Gene 12520 Mouse UniProt ID P35762
Background	TAPA1, or CD81, is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell surface proteins that are characterised by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TAPA1 is a cell surface glycoprotein that is known to complex with integrins. It appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. TAPA1 gene is localized in the tumor suppressor gene region and thus it is a candidate gene for malignancies.
Synonyms	S5.7; TAPA-1; TAPA1; Tetraspanin-28; Tspan-28; TSPAN28
Reference Data

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