Cd68 Rat Monoclonal Antibody [Clone ID: FA-11]

Specifications

Product Data
Clone Name	FA-11
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 106 cells in 100 µl (Membrane permeabilisation is required) . Rat anti mouse CD68 antibody, clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical studies, using both frozen and paraffin-embedded tissues ( Masaki et al. 2003) and Devey et al. 2009). Rat anti mouse CD68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Purified Concanavalin A acceptor glycoprotein from P815 cell line.
Specificity	The antibody recognizes CD68. SM1550R does detect surface Macrosialin at low levels in resident Mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Formulation	PBS Label: PE State: Lyophilized purified IgG fraction from tissue culture supernatant Stabilizer: 1% BSA, 5% Sucrose Preservative: 0.09% Sodium Azide Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1.0 ml distilled water.
Purification	Affinity Chromatography on Protein G
Conjugation	PE
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	CD68 antigen
Database Link	Entrez Gene 12514 Mouse UniProt ID P31996
Background	Macrosialin is a heavily glycosylated transmembrane protein of 87-115kD, which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.
Synonyms	Gp110, Macrosialin, Macrophage marker
Reference Data

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