6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: AD1.1.10]

Specifications

Product Data
Clone Name	AD1.1.10
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA Immunoprecipitation Immunhistology on Frozen Sections.  Western blot: 1/500-1/1000. This antibody is routinely tested on Histidine tagged recombinant protein.
Host	Mouse
Clonality	Monoclonal
Immunogen	PAX6 transcription factor linked to histidine tag.
Specificity	This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, Yeast or insect cells. It reacts against all His tagged proteins so far tested. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or Mammalian cells.
Formulation	PBS containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification.
Reference Data

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