Tumor necrosis factor (TNF-alpha) Rat Monoclonal Antibody [Clone ID: MP6-XT22]

Specifications

Product Data
Clone Name	MP6-XT22
Applications	ELISA, FC, IHC
Recommend Dilution	ELISA: 1-10 µg/ml. Flow Cytometry: Use 10 µl of 1/10-1/50 diluted antibody to label 106 cells in 100 µl (Membrane permeabilisation is required). Immunohistochemistry on Frozen Sections.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Recombinant Murine TNF-alpha expressed in E. coli.
Specificity	This antibody is specific for tumour necrosis factor alpha (TNF alpha). Clone MP6-XT22 is reported to neutralise the effects of Mouse TNF alpha (PMID: 18535177, 7499860). We recommend the use of SM1694LE for neutralizing studies.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor
Database Link	Entrez Gene 21926 Mouse UniProt ID P06804
Background	TNA alpha is a 17 KDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immuno-regulatory role.
Synonyms	TNF, TNF-a, TNFA, TNFSF2, Cachectin
Reference Data

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