CD3E (Cytopl. Dom.) Rat Monoclonal Antibody [Clone ID: CD3-12]

Specifications

Product Data
Clone Name	CD3-12
Applications	FC, IF, IHC, WB
Recommend Dilution	Western Blot. Flow Cytometry: 1/50-1/100 (Membrane permeabilisation is required). Immunohistochemistry on Frozen Sections: 1/1000. Immunohistochemistry on Paraffin Embedded Tissues: 1/1000. Heat antigen retrieval treatment for 20 minutes prior to staining of paraffin sections is required (Tris/EDTA buffer pH 9.0 is recommended for this purpose). Recommended Positive Control Tissue: Tonsil.
Reactivity	Canine, Chicken, Equine, Human, Mouse, Porcine
Host	Rat
Clonality	Monoclonal
Immunogen	Synthetic peptide sequence derived from Cytoplasmic epitope of CD3
Specificity	This antibody recognises a highly conserved epitope of the CD3 molecule expressed by T lymphocytes. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species cross reactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005).
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD3e molecule
Database Link	Entrez Gene 916 Human UniProt ID P07766
Background	T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Synonyms	T3/Leu-4
Reference Data

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