Integrin alpha E (ITGAE) Mouse Monoclonal Antibody [Clone ID: 2G5.1]

Specifications

Product Data
Clone Name	2G5.1
Applications	FC, IHC, IP
Recommend Dilution	Flow cytometry: 1/50 - 1/100; use 10 µl of the suggested working dilution to label 5 x 10e5 cells in 100µl. Immunoprecipitation. Immunohistochemistry on frozen sections: 1/50 - 1/100. Recommended positive control tissue: intestine.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Human Intestinal lymphocyte. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Specificity	This antibody recognises the CD103 cell surface antigen, also known as the alphaE integrin. This reacts with various subsets of intra-epithelial lymphocytes:- 40% of lamina propria T-cells; 30% of mesenteric lymphoblasts and some lymphocytes from other mucosa. It recognises less than 0.1% bone marrow cells and less than 0.7% PBL.
Formulation	PBS containing 0.09% Sodium Azide and 0.1% Bovine Serum Albumin State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha E
Database Link	Entrez Gene 3682 Human UniProt ID P38570
Background	CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150kDa alpha chain and a 120kDa beta chain. Integrin alpha E is expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes.
Synonyms	Integrin alpha-E, Integrin alpha-IEL, HML-1 antigen, ITGAE
Reference Data

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