CD45 (PTPRC) (CD45RB) Mouse Monoclonal Antibody [Clone ID: MEM-55]

Specifications

Product Data
Clone Name	MEM-55
Applications	FC, IHC, IP, WB
Recommend Dilution	Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed. Flow cytometry: Recommended dilution: 0.5-2 μg/ml.
Reactivity	Human, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Human thymocytes and T lymphocytes
Specificity	The antibody MEM-55 recognizes a siliadase-sensitive epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, receptor type C
Database Link	Entrez Gene 5788 Human UniProt ID P08575
Background	CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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