Granzyme B (GZMB) Mouse Monoclonal Antibody [Clone ID: GB7]

Specifications

Product Data
Clone Name	GB7
Applications	IHC, WB
Recommend Dilution	Western blot. Immunohistochemistry on Paraffin Sections: 1/50-1/200. Requires antigen retrieval using heat treatment with Sodium Citrate buffer pH 6.0.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Granzyme B. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Specificity	This antibody recognizes the serine protease Granzyme B, which is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs).
Formulation	TRIS buffered saline, pH 8.0 State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.01% Thiomersal
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	granzyme B
Database Link	Entrez Gene 3002 Human UniProt ID P10144
Background	Granzyme A and Granzyme B are serine proteases that mediate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Both granzyme A and granzyme B are synthesized as inactive proenzymes, and they are stored within cytolytic granules and released by effector cells during degranulation. In activated CTLs, granzyme A and granzyme B are processed and activated by cathepsin C, and they then function to induce apoptosis by two distinct pathways. Granzyme B proteolytically cleaves and activates members of the caspase family of cysteine proteases, including caspase-3, caspase-6, caspase-7 and caspase-9. When cleaved, these caspases assemble into active holoenzymes that then mediate apoptosis through a defined proteolytic cascade involving nuclear lamins and PARP (poly ADP ribose polymerase). Granzyme A mediates the activation of apoptosis by inducing single-strand DNA breaks, membrane perturbation and nuclear condensations in an alternative pathway that is independent from caspase activation or the caspase proteolytic cascade.
Synonyms	Granzyme-2, CGL1, CTSGL1, CSPB, CTLA1, CTLA-1, GRB, SECT, Fragmentin-2
Reference Data

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