Anpep Rat Monoclonal Antibody [Clone ID: R3-63]

Specifications

Product Data
Clone Name	R3-63
Applications	FC
Recommend Dilution	Flow cytometry: neat - 1/5; use 10 µl of the suggested working dilution to label 10e6 cells in 100µl; The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse intestinal APN. Spleen cells from immunised mice were fused with cells of the IR983F rat myeloma cell line.
Specificity	This antibody recognises aminopeptidase N (APN), a cell surface protein homologous with human CD13. Clone R3-63 has been reported to block mouse APN enzyme activity. We recommend the use of SM1856A for this purpose.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Gene Name	alanyl (membrane) aminopeptidase
Database Link	Entrez Gene 16790 Mouse UniProt ID P97449
Background	In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells.
Synonyms	Aminopeptidase N, ANPEP, APN, PEPN
Reference Data

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