p53 (TP53) (Wild type + Mutant) (181-190) Mouse Monoclonal Antibody [Clone ID: DO-11]

Specifications

Product Data
Clone Name	DO-11
Applications	IHC, IP, WB
Recommend Dilution	Western Blot: 1/1000. Immunoprecipitation. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/1000; This product does require antigen retrieval using heat treatment prior to staining of paraffin sections; Sodium citrate buffer pH6.0 is recommended for this purpose. Recommended Positive Control: Colon or breast carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human p53. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Specificity	This antibody recognises the p53 tumour suppressor protein, binding to both wild type and mutant forms. DO-11 recognises an epitope within the central region of p53 between amino acids 181 - 190.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tumor protein p53
Database Link	Entrez Gene 7157 Human UniProt ID P04637
Synonyms	Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13
Reference Data

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