PECAM1 Mouse Monoclonal Antibody [Clone ID: LCI-4]

Specifications

Product Data
Clone Name	LCI-4
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl or 100 µl of lysed whole blood.
Reactivity	Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine CD31/human IgGFc fusion protein
Specificity	This anitbody recognizes CD31, a 140kD cell surface protein also called PECAM-1. Clone LCI-4 has been shown to stimulate tyrosine phosphorylation of CD31 on endothelial cells. Clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmambrane region as demonstrated by human CD31 domain deletion mutant protein binding studies (Nasu et al.1999). Mouse anti Pig CD31, clone LCI-4 immunoprecipitates a protein of ~130kDa from lysates of porcine aortic endothelial cells and is trongly expressed at cell junctions (Nasu et al. 1999).
Formulation	PBS containing 0.09% Sodium Azide, 1% BSA, 5% Sucrose Label: PE State: Lyophilized purified IgG fraction Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1 ml distilled water.
Conjugation	PE
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Database Link	UniProt ID Q95242
Background	Cell adhesion molecules are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play an important role in embryogenesis and development. Neuronal cell adhesion molecule (NCAM) expression is observed in a variety of human tumors including neuroblastomas, rhabdomyosarcomas, Wilms’ tumors, Ewing’s sarcomas and some primitive myeloid malignancies. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the B2α1 and B2αM Integrins. PECAM-1 (platelet/endothelial cell adhesion molecule-1), also referred to as CD31, is a glycoprotein expressed on the cell surfaces of monocytes, neutrophils, platelets and a subpopulation of T cells. VCAM-1 (vascular cell adhesion molecule-1) was first identified as an adhesion molecule induced on human endothelial cells by inflammatory cytokines such as IL-1, tumor necrosis factor (TNF) and lipopolysaccharide (LPS). The KALIG gene encodes a nerve cell adhesion molecule (NCAM)-like protein and is deleted in 66% of patients with Kallmann’s syndrome, anosmia with secondary hypogonadism.
Synonyms	PECAM-1, EndoCAM, GPIIA'
Reference Data

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