Itgb1 Hamster Monoclonal Antibody [Clone ID: HM beta 1.1]

Specifications

Product Data
Clone Name	HM beta 1.1
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat-1/5 diluted antibody to label 10e6 cells in 100 µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by appropriate blocking.
Reactivity	Mouse, Rat
Host	Hamster
Clonality	Monoclonal
Immunogen	Purified mouse VLA-4 antigen. Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.
Specificity	This antibody recognizes the Integrin beta 1 subunit (CD29). Clone HM beta 1.1 is reported to inhibit beta 1 integrin mediated adhesion. (Ref.1) We recommend the use of SM2210LE for this purpose.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative, 1% BSA and 5%Sucrose Label: PE State: Liquid purified IgG fraction Label: R. Phycoerythrin (RPE)
Purification	Affinity Chromatography on Protein G.
Conjugation	PE
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	integrin beta 1 (fibronectin receptor beta)
Database Link	Entrez Gene 16412 Mouse UniProt ID P09055
Background	CD29 is a 110KD cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation.
Synonyms	Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, ITGB1, FNRB, MDF2, MSK12
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.