CD45 / LCA Mouse Monoclonal Antibody [Clone ID: UM16-6]

Specifications

Product Data
Clone Name	UM16-6
Applications	FC
Recommend Dilution	Flow Cytometry: Neat - 1/5; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivity	Chicken
Host	Mouse
Clonality	Monoclonal
Immunogen	COS cells transfected with CD45 gene.
Specificity	This antibody recognizes CD45 (leucocyte common antigen).
Formulation	Containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: PE State: Lyophilized purified IgG Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1.0 ml distilled water
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	PE
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Database Link	UniProt ID Q9I8D7
Background	CD45 is a heavily-glycosylated transmembrane protein tyrosine phosphatase (PTPase) expressed by all nucleated cells of haematopoietic origin. Variation in the expression of a particular CD45 isoform, is regulated during the haematopoietic development of the different cell lineages. CD45 is essential for antigen-induced signal transduction through the antigen receptor and as with other PTPase family members, acts in balance with protein tyrosine kinases, causing the dephosphorylation of negative regulatory tyrosine sites. Studies have indicated that dephosphorylation by CD45, is required for the activation of the src-family kinases p56lck and p59fyn. Investigations into the properties of CD45 in chicken models are limited, but there is evidence of the existence of an additional cysteine residue near the transmembrane region. The overall domain structure between mammalian and chicken CD45 appears to be conserved, but the sequence homology between the extracellular regions is very low.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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