CD34 Mouse Monoclonal Antibody [Clone ID: 1H6]

Specifications

Product Data
Clone Name	1H6
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Reactivity	Canine
Host	Mouse
Clonality	Monoclonal
Immunogen	Canine CD34 fusion protein.
Specificity	SM2257F is specific for Canine CD34, a cell surface antigen of approximately 110 kDa expressed by endothelial cells and haematopoietic stem cells. Clone 1H6 is reported for use in CD34+ enrichment studies (4,5).
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q28270
Background	The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Synonyms	Hematopoietic progenitor cell marker
Reference Data

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