CD62P (SELP) Mouse Monoclonal Antibody [Clone ID: Psel.KO.2.7]

Specifications

Product Data
Clone Name	Psel.KO.2.7
Applications	FC, IHC, IP
Recommend Dilution	Immunoprecipitation. Flow Cytometry: Use 10 µl of 1/25-1/50 diluted antibody to label 106 cells in 100 µl. Immunohistology on Frozen Sections: 1/200 (See Reference 1).
Reactivity	Bovine, Equine, Feline, Goat, Human, Mouse, Rat, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	P-selectin transfected 300.19 cells.
Specificity	This antibody recognises the CD62P cell surface antigen.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	selectin P
Database Link	Entrez Gene 6403 Human UniProt ID P16109
Background	CD62P is a 140 kD glycoprotein expressed by activated platelets and endothelial cells. It plays an important role in adhesive processes between leucocytes and endothelial cells. CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.
Synonyms	SELP, GMRP, GRMP, PADGEM, GMP-140, LECAM3
Reference Data

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