Lag3 Rat Monoclonal Antibody [Clone ID: C9B7W]

Specifications

Product Data
Clone Name	C9B7W
Applications	FC
Recommend Dilution	Flow Cytometry: 1/50 - 1/200; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Murine CD223 Ig fusion protein. Cells from immunised Lewis rats were fused with cells of the Sp/20 myeloma cell line.
Specificity	This antibody recognises lymphocyte activation gene-3 (LAG-3), that is also referred to as CD223. Clone C9B7W recognises an epitope within the D2 domain of CD223. The antibody is reported to block the in vitro function of murine LAG-3 but studies suggest that the antibody does not block binding of LAG-3 to MHC class II. We recommend the use of SM2268LE for this purpose.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	lymphocyte-activation gene 3
Database Link	Entrez Gene 16768 Mouse UniProt ID Q61790
Background	CD223 is a 70kD activation-induced cell surface molecule. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion.
Synonyms	LAG-3, FDC
Reference Data

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