CD11b Mouse Monoclonal Antibody [Clone ID: OX-42]

Specifications

Product Data
Clone Name	OX-42
Applications	FC, FN, IF, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl. Immunoprecipitation. Immunofluorescence. Immunohistochemistry on Frozen Sections: 1/50-1/100. Immunohistochemistry on Paraffin Sections: Clone OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). See Whiteland, J.L. et al. for details. Functionally Assays: Clone OX-42 inhibits complement mediated rosettes. We recommend the use of Low Endotoxin CD11b Antibody Cat.-No SM262LE for use in functional studies. See Robinson, A.P. et al. for details.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
Specificity	This antibody recognizes the Rat equivalent of human CD11b, the receptor for the iC3b component of complement. The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes and microglial cells in the brain.
Formulation	PBS, pH 7.2 State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G of Tissue Culture Supernatant
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q63001
Background	CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.
Synonyms	ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data

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