Itgam Mouse Monoclonal Antibody [Clone ID: WT.3]

Specifications

Product Data
Clone Name	WT.3
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 106 cells in 100 µl.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	FTL-43. Lymph node cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.
Specificity	Clone WT.3 reacts with the rat beta 2 integrin, which is designated CD18. The beta 2 integrin may be associated with the integrin alphaL, alphaX or alphaM subunits.  The antibody  has been shown to inhibit homotypic aggregation of PHA blasts.  It also blocks binding of rat lymphocytes to purified rat ICAM-1.
Formulation	PBS containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG fraction Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity chromatography on Protein A
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Gene Name	integrin subunit alpha M
Database Link	Entrez Gene 25021 Rat UniProt ID Q9JI30
Background	CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.
Synonyms	Integrin beta-2, MFI7
Reference Data

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